Euphorbia hirta L. (EH) (Family: Euphorbiaceae) is a pantropical weed, native to India. The present study aimed to extract leaves of EH for comparative estimation of various phytoconstituents and their effects on antimicrobial activity, procured from Karnataka and West Bengal geographical locations. Dried leaves were estimated for macro and micronutrient along with other heavy metal contents. Methanol and aqueous extracts were prepared using hot maceration method and screened for presence of

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... ious phytochemicals. The results revealed alkaloids, flavonoids, tannins, steroids, glycosides and carbohydrates and phenols in leaves in higher amount collected from West Bengal. Based on that, further total alkaloid, flavonoids and phenolics were estimated and then antibacterial activity was carried out using the agar well diffusion method (Different concentrations of crude drugs such 50 µg/ml, 75 µg/ml, and 100 µg/ml) against two gram-positive bacteria, viz., Bacillus subtilis ATCC 6633, Straphyloccus aureus ATCC 29726, and Pseudomonas aeruginosa ATCC 25619, and Escherichia coli ATCC 8739, a group of gram-negative bacteria that frequently cause enteric infections in humans. Minimum inhibitory concentration (MIC) value ranged from 25 to 100 mg/ml. Interestingly, the activity was dependent on content of elements, type of extract, dose as well as source of the collection of leaves. Resulted higher antimicrobial activity of methanolic extract collected from West Bengal soil zone and confirmed its potential broad spectrum antimicrobial activity due to the positive correlation of content of metal ions and soil nature with the sample.