Original Article Down-regulation of SERPINA3 by miR-613 inhibits melanoma cell proliferation and invasion

Baicheng Qiu, Daqing Luo, Yuqing Wei, Wenhui Pan
2016 Int J Clin Exp Pathol   unpublished
MicroRNAs play critical roles in the development of many kinds of cancers. Serpin Peptidase Inhibitor, clade A member 3 (SERPINA3) was found to be abnormally overexpressed in melanoma tissue biopsies and the high expression of SERPINA3 was associated with poor patient survival. This study aimed to reveal the role of miR-613 in melanoma cell proliferation and its potential mechanisms. The expression of miR-613 was detected in both melanoma and normal tissues as well as the melanoma cell lines.
more » ... anoma cell lines. In A-375 cells transfected with miR-613 mimics, western blot, colony formation assay and invasion assay were performed. SERPINA3, a directly target of miR-613, was verified by luciferase reporter assay and was upregulation to study its function in cell proliferation and invasion. Results showed that MiR-613 was decreased in melanoma tissues and cell lines, in A-375 with the lowest expression. Upregulation miR-613 suppressed A-375 cell proliferation and colony formation. MiR-613 decreased SERPINA3 expression via binding to the 3' untranslated regions (3'-UTRs), while the mutant SERPINA3 could not be regulated. Recovering SERPINA3 resulted in promoting cell proliferation and invasion. This study revealed that miR-613 directly suppresses SERPINA3, which might be one of the mechanisms in inhibiting melanoma cell proliferation and invasion. This research offers a promising therapeutic strategy for treatment melanoma.