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Semiautomated DNA Mutation Analysis Using a Robotic Workstation and Molecular Beacons
Background: Our increasing knowledge of the genetic basis of inheritable diseases requires the development of automated reliable methods for high-throughput analyses. Methods: We investigated the combination of semiautomated DNA extraction from blood using a robotic workstation, followed by automated mutation detection using highly specific fluorescent DNA probes, so-called molecular beacons, which can discriminate between alleles with as little as one single-base mutation. We designed twodoi:10.1093/clinchem/47.4.739 fatcat:pywhl3if7jccrgdbpmbqzc7jea