Standardization of Ayurvedic formulations is an important step for establishment of biological activity, consistent chemical profile, or quality assurance for production and manufacturing of herbal drugs. Most of the pharmaceutical industries are using substitute drugs instead of authentic drugs. Karshyahara Yoga (KY) is an Ayurvedic polyherbal nutritive drug comprising of Vidarikanda (Pueraria tuberose (Willd.) DC.)-1 part, Godhuma (Triticum sativum L.)-1 part, Yava (Hordeum vulgare L.)-1

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... Sita (sugar)-2 parts and Pippali (Piper longum L.)-1/20 th part possessing bhrumhana (nourishing) action. Aims of the study was to standardize Karshyahara Yoga (KY), employing standard testing protocol for AYUSH drugs. Physico-chemical, proximate and chemical fingerprint studies like loss on drying at 105 o C, total ash, acid insoluble ash, water soluble ash, alcohol soluble extractive, water soluble extractive, total fat, crude fibre, total carbohydrate, total protein and HPTLC performed as per standard methodology. Quality indicating physical and chemical tests was done and standard values for KY were recorded. Standardization tests done on KY helped in authenticating the polyherbal preparation and also in ensuring the quality of the same.