The study was carried out during a period of February 2017-July 2018 for the detection of brucellosis in 128 suspected patients with age group range from 17 -69 years, who attended to Baghdad teaching hospitals, that had been examined and defined as suspected cases by specialized physician with the recording of clinical manifestation. The diagnosis done by Rose Bengal test (RBT) method, a blood sample was taken from each patients as well as other 30 healthy control matching in age and gender.

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... e study included measurement of the concentration of Interleukin-23, activity of Acidphosphatase, Copper and Zinc in sera of patients and healthy control. The result indicated that Rose Bengal test positive in 18 cases, 12 with a titer of 1:160 and 6 cases with a titer of 1:320. The Level of IL-23, Acid phosphatase increased significantly, with no statistical difference in copper and zinc concentration in both interval ages of patients sera in comparison with healthy control.