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We demonstrated the feasibility of Raman spectroscopy for remote temperature monitoring within the aqueous humor of the rabbit eye in vivo. Using a confocal Raman spectroscopy system, Raman spectra from 2580 to 3800 cm −1 were recorded in HPLC-grade water and in the aqueous humor of the rabbit eye under in vivo and ex vivo conditions within a temperature range of 14-34°C. The ratio between the integrated Raman intensities of two temperature dependent OHvibrational regions (OH2/OH1) in thedoi:10.1117/1.1911901 pmid:16229634 fatcat:pjq4nxbu4jdenp5qeyv6vpafl4