Antidermatophytic efficacy of Silver Nanoparticles Produced From the fungus Aspergillus terreus

Prema Kulkarni, Rajeev Singh T, Krishnaveni, Ashish Singh, Dattu Singh, Vandana Rathod, Jasmine Mathew, Rathod
2016 International Journal of Pharmacy and Pharmaceutical Science Research   unpublished
Skin infections caused by dermatophytes have become more common recently which are generally observed in immunocompromised individuals. These fungi showed resistance towards the conventional antibiotics used to treat them. Therefore, there is an inevitable and urgent medical need for new antifungal agents. Nanomedicine is an emerging field of science which has grown rapidly over the past few years and has even ventured into the field of clinical medicine. Many studies have shown antimicrobial
more » ... own antimicrobial effects of silver nanoparticles (AgNPs) but the effect of AgNPs on dermatophytes is less explored. Hence, in our studies we assessed the efficacy of these AgNPs on such dermatophytes and obtained encouraging results. In the present study, AgNPs produced from Aspergillus terreus was characterized. Thus produced AgNPs were examined for their effects on dermatophytes using disc diffusion method and MIC values were assessed. Further, synergistic studies were also determined. For all the three dermatophytes, the concentrations used were 300µg/ml, 600µg/ml, 1.2mg/ml and 2.4mg/ml respectively. It was observed that 2.4 mg/ml concentration of AgNPs and ketoconazole are enough to inhibit fungi to the maximum. AgNPs have shown good results with Microsporum gypseum where 62.5µg/ml concentration of AgNPs was enough to show MIC against Microsporum gypseum, while MIC of AgNPs with antibiotic (synergistic) was 31.25µg/ml with the same dermatophyte. These results reveal that not only AgNPs individually can suppress the growth of Microsporum gypseum but also AgNPs with ketoconazole has shown enhanced and good MIC against Microsporum gypseum. Next to Microsporum gypseum was Trycophyton rubrum which has shown MIC of 62.5µg/ml in synergistic effect, while it has shown 125µg/ml with AgNPs as well as with the standard antibiotic. Candida tropicalis has shown MIC of 250µg/ml for AgNPs and 500µg/ml with standard antibiotic. The synergistic effect however showed 125µg/ml with Candida tropicalis. From these results, it can be suggested that AgNPs and antibiotic independently have shown good effect but the combination of these two i.e., synergistic effect of AgNPs and ketoconazole has elevated the effect to the maximum. It can be suggested that if at all antibiotics have to be used for the treatment even though having side effects, when AgNPs along with antibiotics to be used in combination one can downsize the antibiotic to be used along with AgNPs.