Sophoraflavanone G from Sophora pachycarpa Enhanced the Antibacterial Activity of Gentamycin against Staphylococcus aureus

Ali Fakhimi, Mehrdad Iranshahi, Seyed Ahmad Emami, Esam Amin-Ar-Ramimeh, Gholamreza Zarrini, Ahmad Reza Shahverdi
2006 Zeitschrift für Naturforschung C - A Journal of Biosciences  
In this study the enhancement effect of Sophora pachy- carpa roots' acetone extract on the antibacterial activity of gentamycin was evaluated against Staphylococcus au­reus. Disc diffusion and broth dilution methods were used to determine the antibacterial activity of gentamy­cin in the absence and presence of plant extract and its various fractions separated by TLC. A clinical isolate of S. aureus was used as test strain. The active component of the plant extract involved in enhancement of
more » ... enhancement of genta- mycin's activity had Rf = 0.72 on a TLC plate. The spec­tral data (1H NMR. 13CNMR) of this compound re­vealed that this compound was 5,7,2′,4′-tetrahydroxy-8- lavandulylflavanone (sophoraflavanone G), previously isolated from Sophora exigua. In the presence of 0.03 μg/ mL of sophoraflavanone G the MIC value of gentamy­cin for S. aureus decreased from 32 to 8 μg/mL (a four­fold decrease). These results signify that the ultra-low concentration of sophoraflavanone G potentiates the an­timicrobial action of gentamycin suggesting a possible utilization of this compound in combination therapy against Staphylococcus aureus.
doi:10.1515/znc-2006-11-1201 fatcat:aqa4s7lcxzdmxnaligtocjjppm