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An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases
Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein functionality at different cell cycle phases. We apply this technology to the question of how and when Mus81-Mms4 and Yen1 nucleases act on DNA replication or recombination structures. Restriction of Mus81-Mms4 to M phase but not S phase allows adoi:10.7554/elife.52459 fatcat:35m2hdtvbzfqhesioy745wnkjq