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To examine a potential role for soybean phytoestrogens in postmenopausal bone loss, twenty-four 12-weekold Sprague-Dawley rats were divided randomly into 4 groups and given controlled diets for 16 weeks. The treatment groups were as followed: sham operated, ovariectomized (OVX) control, OVX + isoflavone extract (6.25 g/kg), and OVX + 17-estradiol (4 mg/ kg). OVX treatments reduced femoral and fourth lumbar vertebral bone density and mineral content (p < 0:01), decreased uterine weight (p <doi:10.1271/bbb.68.1040 pmid:15170107 fatcat:7uqsy545ind45cavot3fb75tqy