Evaluation of the Preventive Effect of Isoflavone Extract on Bone Loss in Ovariectomized Rats

Yoon-Bok LEE, Hyong Joo LEE, Kang Sung KIM, Jae-Yong LEE, Sang-Yoon NAM, Sang-Hee CHEON, Heon-Soo SOHN
2004 Bioscience, biotechnology and biochemistry  
To examine a potential role for soybean phytoestrogens in postmenopausal bone loss, twenty-four 12-weekold Sprague-Dawley rats were divided randomly into 4 groups and given controlled diets for 16 weeks. The treatment groups were as followed: sham operated, ovariectomized (OVX) control, OVX + isoflavone extract (6.25 g/kg), and OVX + 17-estradiol (4 mg/ kg). OVX treatments reduced femoral and fourth lumbar vertebral bone density and mineral content (p < 0:01), decreased uterine weight (p <
more » ... ne weight (p < 0:01), accelerated body weight increases (p < 0:05), and increased the activities (p < 0:01) of both serum alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP). Supplementation with isoflavone prevented the losses of bone density and mineral content caused by OVX (p < 0:01). Although both isoflavone and 17estradiol exhibited similar bone-sparing ability on the OVX-induced bone loss, the effect of isoflavone was not the same as that of 17-estradiol on the serum ALP and TRAP, body weight increase, and uterine weight change. We concluded that dietary supplementation with soybean isoflavone can prevent postmenopausal bone loss via a different mechanism of estrogen in OVX rats.
doi:10.1271/bbb.68.1040 pmid:15170107 fatcat:7uqsy545ind45cavot3fb75tqy