Effect of bionic electrical stimulation on the differentiation of embryonic stem cells into cardiomyocytes in the presence myocardial cells in vitro

Li-na ZHENG, Xiao-gang ZHANG, Liang YANG, Hui ZHANG, Jia-nan WANG
2011 Medical Journal of Chinese People's Liberation Army  
Objective To investigate the effects of electrical stimulation on the differentiation of embryonic stem cells(ESCs) into cardiomyocytes in the presence of myocardial cells in vitro.Methods ESCs and neonate rat cardiomyocytes were isolated and cultured.These cells of primary culture were divided into 5 groups according to whether or not electric stimulation was given and the presence of cardiomyocytes: control group,stimulation group,cardiomyocytes group,stimulation+ cardiomyocyte conditioned
more » ... ium group,and stimulation+cardiomyocytes group.Expression of troponin T(cTnT) in the differentiated cells from ESCs was examined by immunofluoresence on the 5th,7th and 14th day.Results In the group co-cultured with myocardial cell and electrical stimulation,the differentiating ratio of cardiomyocytes derived from ESCs and expressing cTnT was 40.00%±2.39%,and it was higher than that in control group(2.00%±1.60%),stimulation group(3.00%±2.00%),cardiomyocytes group(28.70%±4.06%),stimulation+cardiomyocyte conditioned medium group(17.10%±2.23%,P < 0.05).Conclusion Bionic electric stimulation promotes the differentiation of ESCs into cardiomyocyte in a microenvironment consisting of myocardial cells.
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