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A cyanide biosensor based on a pH-sensitive p-doped electrolyte-insulatorsemiconductor (EIS) structure with an immobilised enzyme (cyanidase) is realised at the laboratory scale. The immobilisation of the cyanidase is performed in two distinct steps: first, the covalent coupling of cyanidase to an N-hydroxysuccinimide-(NHS) activated Sepharose TM gel and then, the physical entrapment of NHS-activated Sepharose TM with the immobilised cyanidase in a dialysis membrane onto the EIS structure. Thedoi:10.3390/s7081415 fatcat:ejthynzx7neprjjhbbrskbcsum