EVALUATION OF ANTIOXIDANT, CYTOTOXIC AND CYTOPROTECTIVE ACTIVITIES OF ALHAGI MAURORUM EXTRACTS AND PHYTOCHEMICAL PROFILE

Ataa Said, Mohamad Abo-El-Fotouh, Abdel-Rahman Alshabrawy, Jitka Psotova, Usama Hawas, Amira Omar, Khaled Rashed, Ataa Said, Khaled Rashed
2014 Indian Journal of Drugs   unpublished
This study was carried out to evaluate antioxidant, cytotoxic and cytoprotective activities of Alhagimaurorum petroleum ether, diethyl ether, chloroform and methanol (70%) extracts and to investigate the main phyto-constituents. Antioxidant activity was carried out by measuring free radical scavenging on model reaction with stable 2, 2-diphenyl-1-picrylhydrazyl radical. Cytotoxic effect was carried out on both primary rat hepatocyte culture, and hepatoma cell line, as well for cytoprotective
more » ... r cytoprotective effect against lipid peroxidation and against oxidantive stress induced by hydrogen peroxide. The results showed that diethyl ether is the best active free radical scavenging agent and it showed IC50 0.083 ± 0.01 (mg/mL). Ether extract showed the highest cytotoxic effect while methanol extract was the least. Concerning cytoprotective effect, all the extracts showed a mild cytoprotective effect. Phytochemical analysis of the extracts proved the presence of triterpenes in petroleum ether, ether and chloroform extracts. Flavonoids, triterpenes and carbohydrates were detected in methanol 70% extract. In conclusion this study may help to discover new natural antioxidant, cytotoxic and cytoprotective substances that could serve as selective agents for infectious diseases.
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