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Prenatal development of rat thyroid C-cells was immunocytochemically investigated in culture. Immunoreactivities for calcitonin (CT) and CT gene-related peptide (CGRP) were used as the markers of C-cell differentiation. The thyroid glands at various gestational ages were enzymatically dispersed and cultured in Dulbecco's modified Eagle medium supplemented with 5% fetal calf serum and 4% rat embryo extract. In the dispersed thyroid cells of day 14 embryos, CT-and CGRP-immunoreactive cellsdoi:10.2220/biomedres.9.413 fatcat:dhmjfj3p6zcqbmgtdiftvtoswq