Soroprevalência de Anaplasma marginale em bovinos leiteiros e estudos sobre a dinâmica da infecção natural em bezerros holandeses no Sul do Brasil

Gisele Maria de Andrade, Odilon Vidotto, Marilda Carlos Vidotto, Eidi Yoshihara, Flora S. Kano, Carlos Henrique S. Amaral
2004 Semina: Ciências Agrárias  
Sera of 708 animals (cows, heifers and calves) from 13 dairy herds in the Londrina region of Paraná, Brazil, were tested for antibodies to Anaplasma marginale by a competitive ELISA assay (cELISA). Ten 2 to 20 days old Holstein calves, from one of the 13 herds studied, were monitored during one year. Blood samples from each calf were collected monthly and tick burden counting was performed every fortnight. Percentage of infected erythrocytes was established by Giemsa-stained smears, and sera
more » ... smears, and sera samples were examined by cELISA to detect antibodies against A. marginale. In the 13 herds, 92.94% of the animals were seropositive to A. marginale, which indicates that Londrina is an area of enzootic stability. Among the three animal categories (cows, heifers and calves), the rates were 98.29%, 96.64% and 81.25%, respectively. Passive transfer of maternal antibodies to calves was demonstrated by cELISA. From ten calves, nine (90%) were seropositive at the first sampling, revealing colostral antibodies anti-A. marginale. These antibodies remained in calves for 2 to 3 months. After this period the calves were infected with ticks, and then all of them were seropositive to Anaplasma. Five 4 to 7 months old calves showed rickettsemia ranging from 0.1% to 3.8%. Two of them were treated with tetracycline. The rickettsemia and clinical signs of anaplasmosis of these calves were coincident with tick burden increase.
doi:10.5433/1679-0359.2001v22n2p155 fatcat:2ag2bxby2bawrkzlsbmmhi7ipu