Increasing specificity of modern test-systems for diagnosis of ToRCH infections on example of IgM CMV

S. S. Vashukova
2019 Medical alphabet  
The aim of the study was to Substantiate the format of tests for laboratory diagnosis of ToRCH-infections, to identify the causes of insufficient sensitivity and specificity of the ELISA tests on the example of determination of immunoglobulin M against cytomegalovirus, and to offer the solutions. Conclusions. The method of mucapture solves the problem of specificity, and the variant of sequential incubation with conjugates - the first incubation with Biotin, the second with streptavidin -
more » ... es the signal by increasing the number of covalent bonds, which increases the sensitivity of the test.
doi:10.33667/2078-5631-2019-1-4(379)-46-48 fatcat:e3vaondgnretjbet7pzxyped4i