Simultaneous Measurement of Three Tocopherols, All-trans-retinol, and Eight Carotenoids in Human Plasma by Isocratic Liquid Chromatography

Z. Liu, H.-J. Lee, F. Garofalo, D. J. A. Jenkins, A. El-Sohemy
2011 Journal of Chromatographic Science  
We describe a simplified isocratic HPLC method for the simultaneous determination of all-trans-retinol, 3 tocopherols (α-, δ-, and γtocopherol), and 8 carotenoids (lutein, zeaxanthin, canthaxanthin, β-cryptoxanthin, all-trans-lycopene, 5-cis-lycopne, α-carotene, and β-carotene) in human plasma, which uses a single C18 reversed-phase column (4.6 × 250 mm, 3 µm). A photodiode array detector was used to measure the UV-vis wavelength absorbance of retinol and the eight carotenoids, and fluorescence
more » ... detection was used for the tocopherols. The linear ranges of the calibration curves for the calibration solutions injected into the HPLC column are 0.02-6.0 µg/mL for all-trans-retinol, β-cryptoxanthin and α-carotene; 0.01-3.0 µg/mL for δ-tocopherol, lutein, and lycopene; 0.08-24.0 µg/mL for γ-tocopherol; 0.3-90.0 µg/mL for α-tocopherol; 0.005-1.5 µg/mL for zeaxanthin and canthaxanthin; and 0.04-12.0 µg/mL for β-carotene.
doi:10.1093/chrsci/49.3.221 fatcat:lje4xztvr5gcxd25bx6t6h5si4