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Unravelling the mechanism of non-ribosomal peptide synthesis by cyclodipeptide synthases
2014
Nature Communications
Cyclodipeptide synthases form cyclodipeptides from two aminoacyl transfer RNAs. They use a ping-pong mechanism that begins with transfer of the aminoacyl moiety of the first aminoacyl tRNA onto a conserved serine, yielding an aminoacyl enzyme. Combining X-ray crystallography, site-directed mutagenesis and affinity labelling of the cyclodipeptide synthase AlbC, we demonstrate that the covalent intermediate reacts with the aminoacyl moiety of the second aminoacyl tRNA, forming a dipeptidyl
doi:10.1038/ncomms6141
pmid:25284085
fatcat:gu4wqyywkzgq5lh45tl4kw7enq