Unravelling the mechanism of non-ribosomal peptide synthesis by cyclodipeptide synthases

Mireille Moutiez, Emmanuelle Schmitt, Jérôme Seguin, Robert Thai, Emmanuel Favry, Pascal Belin, Yves Mechulam, Muriel Gondry
2014 Nature Communications  
Cyclodipeptide synthases form cyclodipeptides from two aminoacyl transfer RNAs. They use a ping-pong mechanism that begins with transfer of the aminoacyl moiety of the first aminoacyl tRNA onto a conserved serine, yielding an aminoacyl enzyme. Combining X-ray crystallography, site-directed mutagenesis and affinity labelling of the cyclodipeptide synthase AlbC, we demonstrate that the covalent intermediate reacts with the aminoacyl moiety of the second aminoacyl tRNA, forming a dipeptidyl
more » ... and identify the aminoacyl-binding sites of the aminoacyl tRNAs.
doi:10.1038/ncomms6141 pmid:25284085 fatcat:gu4wqyywkzgq5lh45tl4kw7enq