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We report the first direct observation of the oxygen-isotope (^16O/^18O) effect on the in-plane penetration depth λ_ab in a nearly optimally doped YBa_2Cu_3O_7-δ film using the novel low-energy muon-spin rotation technique. Spin polarized low energy muons are implanted in the film at a known depth z beneath the surface and precess in the local magnetic field B(z). This feature allows us to measure directly the profile B(z) of the magnetic field inside the superconducting film in the Meissnerdoi:10.1103/physrevlett.92.057602 pmid:14995343 fatcat:atpaymfizzeslbyrk4wpgb7rjq