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The nitrifying bacteria acting on various biofilter substrates including: scoria, P.V.C., marble and zeolite were evaluated. The experiment started with manufacture of a recirculation system with 130 liter fresh water volume in 7 l.min-1 flow rate in 3 replicate for every treatment. The volume of each biofilter was 60×40×30cm and after achievement to stable environmental conditions the nitrifying bacteria at a concentration of 10 5 ml-1 were inoculated in each biofilters. Daily 3-10mg.l-1 offatcat:6uqtu4qnd5cotisbkpc6cehx34