Advances in grape phenotyping: pigment characterization by reflectance and theoretical chemistry

L Rustioni, L Rocchi, F Di Meo, O Failla, P Trouillas
2015 Vitis   unpublished
optical studies of pigment characterization developed by our research team over the past years. Material and Methods: Pigment optical properties have been studied by theoretical chemistry and reflectance spectroscopy. Anthocyanin absorption was described by quantum chemistry calculations based on time-dependent density functional theory (TD-DFT). Further methodological details are reported in RUSTIONI et al. 2013a. Aiming at a grape color classification, berries were studied by a Lambda 35
more » ... by a Lambda 35 UV-vis spectrophotometer (Perkin Elmer, Waltham, Massachusetts), completed by a Spectralon integrating sphere (as described in RUSTIONI et al. 2013b). To elucidate the sunburn physiology with the purpose of resistant cultivar selection, chlorophylls and melanin-like pigments were studied by a Jaz System spectrometer (Ocean Optics) (RUSTIONI et al. 2014).