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Journal of Virology
The expression vectors pINIII-A and pINIII (Ipp p5) were used to construct plasmids which direct the synthesis in Escherichia coli of the Kirsten ras viral (v-Ki-ras) and human cellular (c-Ki-ras) oncogene products as fusion proteins containing 9 and 10 extra amino acids, respectively, at their N termini. Authenticity of the bacterially produced proteins was determined by immunoprecipitation and immunoblot analyses with ras-specific monoclonal antibodies. After induction withdoi:10.1128/jvi.61.2.302-307.1987 fatcat:kazqzbews5baxaznylf5cgak4q