Structures and disulfide cross-linking of de novo designed therapeutic mini-proteins

Daniel-Adriano Silva, Lance Stewart, Kwok-Ho Lam, Rongsheng Jin, David Baker
2018 The FEBS Journal  
Recent advances in computational protein design now enable the massively parallel de novo design and experimental characterization of small hyperstable binding proteins with potential therapeutic activity. By providing experimental feedback on tens of thousands of designed proteins, the design-build-test-learn pipeline provides a unique opportunity to systematically improve our understanding of protein folding and binding. Here, we review the structures of mini-protein binders in complex with
more » ... fluenza hemagglutinin and Bot toxin, and illustrate in the case of disulfide bond placement how analysis of the large datasets of computational models and experimental data can be used to identify determinants of folding and binding. De novo miniprotein design and stabilization D.-A. Silva et al.
doi:10.1111/febs.14394 pmid:29389072 pmcid:PMC6001749 fatcat:sypsjomfmncgnl6jiare3bomji