CRY-BARs: Versatile light-gated molecular tools for the remodeling of membrane architectures [article]

Anna I Wurz, Wyatt P Bunner, Erzsebet M Szatmari, Robert M Hughes
2022 bioRxiv   pre-print
BAR (Bin, Amphiphysin and Rvs) protein domains are responsible for the generation of membrane curvature and represent a critical mechanical component of cellular functions. Thus, BAR domains have great potential as components of membrane-remodeling tools for cell biologists. In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (CRY-BARs) with applications in the remodeling of membrane architectures and the control of cellular
more » ... dynamics. By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions. Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity. Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function. Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
doi:10.1101/2022.01.28.478241 fatcat:t5nj2uujyza7bfwhctfz2iilsy