Interaction of two cytoplasmic erythroid differentiation-inducing factors in mouse erythroleukemia cells

T Watanabe, M Oishi
1992 Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research  
Our previous cell fusion experiments have suggested that the in vitro erythroid differentiation of mouse erythroleukemia cells is the result of a synergistic reaction involving two intracellular differentiation-inducing factors (DIF); these were subsequently demonstrated in the cytoplasmic fraction of mouse erythroleukemia cells. Here, we present experimental evidence indicating that, under conditions in which the two factors (DIF-I and DIF-II) are coinduced, a new factor, which can trigger
more » ... hroid differentiation upon introduction into undifferentiated mouse erythroleukemia cells, is produced in the cells. A similar factor was also generated in vitro after the incubation of partially purified DIF-I and DIF-II. We found that protein phosphatases could substitute for DIF-II. These and other experiments suggest that protein dephosphorylation at a tyrosine residue(s) is involved in the generation of the new factor.
pmid:1472466 fatcat:4gnkg7aa2jdupe5wjijpxmpmsm