Effects of monoclonal antibody directed to LeY on implantation in the mouse

X. Wang
1998 Molecular human reproduction  
The role of carbohydrates in embryo implantation in the mouse was investigated using an embryo transfer model and a blastocyst-uterine epithelial cell co-culture system. The monoclonal antibody (mAb) AH 6 directed to Le Y oligosaccharide (Fuc α1-2 Gal β 1-4 [Fuc α1-3] GlcNAc) and other three mAbs directed to carbohydrates whose structures are closely related to Le Y were used to show the effect of carbohydrate specificity on implantation. In the embryo transfer model, donor blastocysts (4 days
more » ... ost-coitus) were pretreated with mAb AH 6 (experimental) or other mAbs (control) and transferred into one uterine horn of a recipient. The implantation rate was checked after 5 days. Implantation was significantly inhibited by mAb AH 6 pretreatment, and inhibition was not observed in control groups. In the co-culture system, the attachment and outgrowth rate of blastocysts on the surface of uterine epithelial cells was significantly inhibited when monolayer epithelial cells or blastocysts were pretreated with mAb AH 6 . The most obvious effect of mAb AH 6 was obtained during 2-4 h co-incubation. No inhibition was observed in the control groups. It was, therefore, concluded that oligosaccharide Le Y recognized by mAb AH 6 plays an essential role at the initial stage of implantation. It may act as a mediator molecule for adhesion between the surface of blastocyst and epithelial cell, and its function is carbohydrate-specific.
doi:10.1093/molehr/4.3.295 pmid:9570276 fatcat:z7q6qswzlncf7o6xtgxktbimqe