Lipoprotein Analysis Using Agarose Gel Electrophoresis and Differential Staining of Lipids

Toshimi Kido, Hideaki Kurata, Akiyo Matsumoto, Rie Tobiyama, Toshiaki Musha, Katsuji Hayashi, Seiichi Tamai, Kazunori Utsunomiya, Naoko Tajima, Noel Fidge, Hiroshige Itakura, Kazuo Kondo
2001 Journal of atherosclerosis and thrombosis  
We established a strategy to directly measure cholesterol and triglyceride levels of each lipoprotein fraction using a combination of agarose gel electrophoresis and differential staining. The cholesterol and triglyceride levels determined by electrophoresis correlated significantly with those of ultracentrifugation. The correlation coefficients between these methods were, for cholesterol levels 0.975(very low density lipoproteins, VLDL), 0.986(low density lipoproteins, LDL) and 0.965(high
more » ... and 0.965(high density lipoproteins, HDL) and for triglyceride levels 0.994(VLDL), 0.963(LDL) and 0.959(HDL) respectively. Both intra-and inter-assays showed low values of coefficients of variation (CV) (less than 3.57%). We observed a strong linearity between staining and triglyceride concentration. An increased VLDL-cholesterol was observed in type Ill subjects, a result which enabled distinction between type Ill and type Ilb or type V lipoproteinemia. The method revealed lipoprotein patterns in some samples otherwise unexpected from their corresponding serum lipid parameters. Analyses of these electrophoretic patterns thus provide an effective technique to classify types of hyperlipidemia defined by the WHO. Furthermore, quantitative measurement of chylomicrons, usually difficult, proved to be achievable, providing an additional analysis of postprandial hyperlipidemia and the exact measurement of LDL-cholesterol after diet. Consequently, we recommend this simple and easy method for clinical evaluation of abnormalities in lipoprotein profiles.
doi:10.5551/jat1994.8.7 pmid:11686314 fatcat:o47e23skwzaptjdmknv4v2nuf4