Pengaruh Ekstrak Etanol Propolis Terhadap Aktivitas dan Kapasitas Fagositosis pada Kultur Makrofag yang Diinfeksi Enteropathogenic Escherichia coli (EPEC)

Iis Herawati, Usep A. Husin, Sunarjati Sudigdoadi
2015 Majalah Kedokteran Bandung  
Abstrak Penanganan penyakit infeksi diperlukan suatu imunostimulator. Propolis adalah campuran resin yang dikumpulkan lebah dari tumbuh-tumbuhan, digunakan sebagai material isolasi sarang lebah, merupakan bahan yang berpotensi sebagai imunostimulator. Penelitian dilakukan di Laboratorium Penelitian dan Pengujian Terpadu Universitas Gadjah Mada pada Januari-Maret 2013. Penelitian ini bertujuan menganalisis peningkatan aktivitas dan kapasitas fagositosis kultur makrofag yang diberi ekstrak etanol
more » ... beri ekstrak etanol propolis terhadap Enteropathogenic Escherichia coli (EPEC). Desain penelitian eskperimen. Ke dalam kultur makrofag yang diperoleh dari tiga orang subjek, masing-masing diberi ekstrak etanol propolis 6,25; 12,5; 25; 50, dan 100 µg/mL serta kontrol tanpa ekstrak etanol propolis. Inkubasi dilakukan satu hari. Kultur diinfeksikan EPEC selama 30 menit, diwarnai dengan Giemsa dan diamati dengan mikroskop. Persentase aktivitas fagositosis diperoleh dengan menghitung jumlah makrofag yang aktif dalam 100 makrofag. Nilai kapasitas fagositosis diperoleh dengan menghitung jumlah bakteri yang difagosit oleh 50 makrofag. Uji analysis of variance (ANOVA) menunjukkan perbedaan signifikan aktivitas maupun kapasitas fagositosis kultur makrofag yang diberi ekstrak etanol propolis dibanding dengan kontrol (p=0,000). Uji Tukey HSD pada aktivitas fagositosis menunjukkan hasil signifikan antara kontrol dan konsentrasi 6,25 µg/mL (p=0,008), sedangkan pada kapasitas fagositosis terlihat antara kontrol dan konsentrasi 12,5 µg/mL (p=0,001). Simpulan, ekstrak etanol propolis meningkatkan aktivitas dan kapasitas fagositosis kultur makrofag terhadap EPEC dengan konsentrasi minimum 12,5 µg/mL. [MKB. 2015;47(2):102-8] Kata kunci: Aktivitas fagositosis, Enteropathogenic Escherichia coli (EPEC), kapasitas fagositosis, makrofag, propolis Abstract The use of immunostimulant for infectious diseases treatment is needed. Propolis is a mixture of resin collected from plants by bees and used as an insulating material in beehives which has a potential as immunostimulant. This study was performed at . This experimental research aimed to analyze the increase of phagocytosis activity and capacity on macrophages culture added by propolis ethanolic extract against Enteropathogenic Escherichia coli (EPEC). The design of this study was experimental study. Macrophage cultures obtained from three subjects were individually culture supplemented with the 6.25, 12.5, 25, 50, and 100 µg/mL propolis ethanolic extract. A control without the addition of propolis ethanolic extract is also used. Cultures were incubated for one day. Macrophage cultures were infected by EPEC for 30 minutes, stained by Giemsa, and observed by microscope. Percentage of phagocytosis activity is determined by the number of active macrophages per 100 macrophages. The phagocytosis capacity value was determined by counting the number of bacteria that were phagocytized by 50 macrophages. Analysis of variance (ANOVA) showed a significantly different phagocytosis activity and capacity between macrophage cultures added by propolis ethanolic extract and control (p=0.000). Tukey HSD analysis showed significantly different phagocytosis activity between control and subject with a concentration of 6.25 µg/mL (p=0.008) whereas the difference in phagocytosis capacity was seen between control and subject with a concentration of 12.5 µg/mL (p=0.001). In conclusion, propolis ethanolic extract is able to increase the phagocytosis activity and capacity of macrophages against EPEC with a minimum concentration of 12.5 µg/mL. [MKB. 2015;47(2):102-8]
doi:10.15395/mkb.v47n2.460 fatcat:7ed2vr5y4nbcfnmhbsiyfa6tqm