Inactivation of kinase cascades in mesangial cells grown on collagen type I

Tiho Miralem, Douglas M. Templeton
1998 AJP - Renal Physiology  
Miralem, Tiho, and Douglas M. Templeton. Inactivation of kinase cascades in mesangial cells grown on collagen type I. Am. J. Physiol. 275 (Renal Physiol. 44): F585-F594, 1998.-Growth on collagen type I gels is known to suppress the mitogenic responsiveness of mesangial cells. Because these cells proliferate in some renal diseases and themselves synthesize collagen type I, we examined the influence of growth on collagen upon several kinase signaling cascades involved in mesangial cell
more » ... on. Quiescent mesangial cells grown on collagen type I and then stimulated with serum showed a markedly diminished induction of the protooncogene c-fos, compared with their counterparts on plastic or fibronectin. This effect was accompanied by decreased activation of mitogen-activated (Erk family) and Ca 2ϩ / calmodulin-dependent protein kinases. Cells on collagen showed lower basal protein kinase C (PKC) activity and diminished levels of PKC-␣ and -isoforms. Global phosphorylation of tyrosine residues was diminished on collagen, and tyrosine phosphorylation of Erk and focal adhesion kinase in response to serum was not detected, in contrast to cells on plastic. We conclude that attachment of mesangial cells to collagen type I results in a broad suppression of protein phosphorylation that is reflected in diminished induction of the c-fos gene and probably underlies the conversion of cultured mesangial cells to a nonproliferative phenotype. extracellular matrix; c-fos; mitogen-activated protein kinase; calcium/calmodulin-dependent kinase; protein kinase C The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
doi:10.1152/ajprenal.1998.275.4.f585 fatcat:5njsl6kwazambcec46azanfesq