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A new water-soluble reactive perylene tetracarboxylic diimide derivative (PDI-pfp) is designed and synthesized that can realize fast imaging of the endoplasmic reticulum in living cells. The PDI-pfp comprises three functional moieties: perylene tetracarboxylic diimide as fluorescent backbone, poly(ethylene glycol) for providing good water disperse ability, and pentafluorophenol active ester as the reactive group under physiological condition. On the basis of covalent reaction between the activedoi:10.1021/acsomega.8b01275 pmid:31459000 pmcid:PMC6644429 fatcat:bo4gh6vhgrgwdj34xmcxt4zufy