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Protein over-expression in Escherichia coli triggers adaptation analogous to antimicrobial resistance
2021
Microbial Cell Factories
Background The E. coli pET system is the most widely used protein over-expression system worldwide. It relies on the assumption that all cells produce target protein and it is generally believed that integral membrane protein (IMP) over-expression is more toxic than their soluble counterparts. Results Using GFP-tagged proteins, high level over-expression of either soluble or IMP targets results in > 99.9% cell loss with survival rate of only < 0.03%. Selective pressure generates three
doi:10.1186/s12934-020-01462-6
pmid:33430875
fatcat:exmevmyqtjhirbpxyu46m23umm