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ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI

Agustina Setiawati, Sri Hartati Yuliani, Enade Perdana Istyastono, Michael Rahardja Gani, Evy Fenny Veronica, Dina Christin Ayuning Putri, Reza Eka Putra, David Chandra Putra, Agnes Mutiara Kurniawan
2014 Jurnal Farmasi Sains dan Komunitas  

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Abstract: Rapid and simple quantitative analysis isoflavones tempe using densitometric TLC has been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5: 0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distance of 1 μL sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linear relationship obtained at 0.08 - 2 μg/spot with r= 0.9986. The LOD and LOQ of isoflavone were 0.014 μg/spot and 0.048 μg/spot. Genistein
more » ... ained in tempe was 0.151± 0.005 % b/b. Keywords: tempe, isoflavon, TLC
doi:10.24071/jpsc.11180 doaj:b3b57349a64f4d7f845263f20d74c01c fatcat:4fe7fh44uvdwhap3jcfdz7vbum
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Agustina Setiawati, Sri Hartati Yuliani, Enade Perdana Istyastono, Michael Rahardja Gani, Evy Fenny Veronica, Dina Christin Ayuning Putri, Reza Eka Putra, David Chandra Putra, Agnes Mutiara Kurniawan. "ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI." Jurnal Farmasi Sains dan Komunitas (2014)