The objective of this is report was to evaluate the physicochemical characteristics and the antibacterial activity of honey produced for human consumption in some counties in Amazon, Cerrado and Pantanal of Mato Grosso, Brazil. Honey samples were collected in 6 municipalities, totaling 18 samples, 3 samples per municipality. The physicochemical characteristics evaluated were moisture, reducing sugars, apparent sucrose, glucose and fructose by HPLC system, ashes, insoluble solids, free acidity,

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... MF, diastase activity, Lund, Fiehe and Lugol reactions, electrical conductivity and color. The content of total phenolic and flavonoids were quantified using standard curve of gallic acid and quercetin. The antibacterial activity of honey was evaluated by disk diffusion to five bacteria. Fifteen out of eighteen samples were reproved in some of the physicochemical characteristics. Honeys' average content of total phenolic was 35.68 mg GAE/100 g of honey. The content of total phenolic and flavonoids showed positive correlation with the color of the honeys. The studied honeys did not present antibacterial activity, but the phenolic and flavonoid contents originate potential antioxidant and prebiotic for the darkest honeys. Mato Grosso has potential to the production of the floral honey, in need of adequacy on the quality of the honey to the physicochemical characteristic's patterns.