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Immunodetection of human IgG anti-Toxocara canis was developed based on ELISA and on the use of polysiloxane/ polyvinyl alcohol (POS/PVA) beads. A recombinant antigen was covalently immobilized, via glutaraldehyde, onto this hybrid inorganic-organic composite, which was prepared by the sol-gel technique. Using only 31.2 ng antigen per bead, a peroxidase conjugate dilution of 1:10,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. This procedure is comparabledoi:10.1590/s0074-02762003000300018 fatcat:a47hhyow2rbu5da4per5vhpbua