Characterization of Bacillus polyfermenticus KJS-2 as a Probiotic

Kang Min Kim
2009 Journal of Microbiology and Biotechnology  
The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be 60.71±4.9%. In addition, treatment of B. polyfermenticus KJS-2 with artificial gastric juice (pH 2.0) and bile acid (pH 6.5) for 4 h resulted in a final viability of 140±7.9% and 108±3.5%,
more » ... 7.9% and 108±3.5%, respectively. Finally, the results of adhesion experiments using Caco-2 cells revealed that the adherence of B. polyfermenticus KJS-2 to Caco-2 cells was approximately 65±0.6%.
doi:10.4014/jmb.0903.113 fatcat:ziasudtjvvflxp7ewtvthr7nj4