Diallyl disulfide induces Ca2+ mobilization in human colon cancer cell line SW480

Chung-Yi Chen, Chien-Fu Huang, Ya-Ting Tseng, Soong-Yu Kuo
2011 Archives of Toxicology  
Diallyl disulfide (DADS), one of the major organosulfur compounds of garlic, is recognized as a group of potential chemopreventive compounds. In this study, we examines the early signaling effects of DADS on human colorectal cancer cells SW480 loaded with Ca 2? -sensitive dye fura-2. It was found that DADS caused an immediate and sustained rise of [Ca 2? ] i in a concentration-dependent manner (EC 50 = 232 lM). DADS also induced a [Ca 2? ] i elevation when extracellular Ca 2? was removed, but
more » ... e magnitude was reduced by 45%. Depletion of intracellular Ca 2? stores with 2 lM carbonylcyanide m-chlorophenylhydrazone, a mitochondrial uncoupler, didn't affect DADS's effect. In Ca 2? -free medium, the DADS-induced [Ca 2? ] i rise was abolished by depleting stored Ca 2? with 1 lM thapsigargin (an endoplasmic reticulum Ca 2? pump inhibitor). DADS-caused [Ca 2? ] i rise in Ca 2? -containing medium was not affected by modulation of protein kinase C activity. The DADS-induced Ca 2? influx was blocked by nicardipine (10 lM). U73122, an inhibitor of phospholipase C, abolished ATP (but not DADS)-induced [Ca 2? ] i rise. These findings suggest that DADS induced a significant rise in [Ca 2? ] i in SW480 colon cancer cells by stimulating both extracellular Ca 2? influx and thapsigargin-sensitive intracellular Ca 2? release via as yet unidentified mechanisms.
doi:10.1007/s00204-011-0748-4 pmid:21879349 fatcat:vioxfskl3vaadf5tzix7luhkhe