A Multimode Optical Imaging System for Preclinical Applications In Vivo: Technology Development, Multiscale Imaging, and Chemotherapy Assessment

Jae Youn Hwang, Sebastian Wachsmann-Hogiu, V. Krishnan Ramanujan, Julia Ljubimova, Zeev Gross, Harry B. Gray, Lali K. Medina-Kauwe, Daniel L. Farkas
2011 Molecular Imaging and Biology  
Purpose-Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. Procedures-We integrated several optical imaging technologies,
more » ... fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. Results-The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. Conclusions-This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.
doi:10.1007/s11307-011-0517-z pmid:21874388 pmcid:PMC3487699 fatcat:k4br3dpmcjbt5e7vpqqi5smrby