Preliminary Studies On Production And Partial Purification Of Toxins Associated With Black Tar Disease Of Yam (Dioscorea Species) In Makurdi, Benue State, Nigeria

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2013 IOSR Journal of Environmental Science Toxicology and Food Technology  
Studies were carried out to identify pathogens and toxins associated with the black tar disease of yam foliage (Yam anthracnose) in Makurdi, Benue State, Nigeria. Infected yam leaves and stems showing symptoms were collected from farms and cultured for isolation of causal organisms. Pure cultures of isolates were obtained using single spore method. Toxin extractions were done using 100ml of ethyl acetate in separating funnels. These were dried using a vacuum rotator evaporator. Partial
more » ... ion of the toxin was done with the use of thin layer chromatography (TLC) plates. Results showed the predominant presence of Colletotrichum gloeosporioides. TLC quantification in four solvent systems suggests that the toxin produced is a glycoprotein.
doi:10.9790/2402-0338589 fatcat:xprp7f2puzhtdeoo5bl2u53pcm