Rolle der Relaxin-artigen Peptide in der Tumorbiologie der Schilddrüse [article]

Sabine Hombach-Klonisch, Martin-Luther Universität, Universitäts- Und Landesbibliothek Sachsen-Anhalt
2018
The polypeptide hormones relaxin and INSL3, members of the superfamily of insulin-like growth factors, are differentially expressed in the human thyroid. INSL3 is expressed in hyperplastic thyrocytes of Graves disease and in neoplastic thyrocytes of human papillary, folliculary and anaplastic thyroid carcinoma. Relaxin is a novel marker for neoplastic human thyrocytes. Normal thyrocytes are devoid of both hormones. Relaxin binds to both of the G-protein-coupled receptors, LGR7 and LGR8, whereas
more » ... 7 and LGR8, whereas INSL3 exclusively binds to LGR8. Expression of both receptors is upregulated in thyroid carcinoma tissue suggesting relaxin-like peptides to act in an auto-/ paracrine way. Transfectants of the human follicular thyroid carcinoma cell line FTC-133 stably producing and secreting bioactive relaxin or INSL3, respectively, were generated to investigate the cellular effects of relaxin-like peptides. Relaxin increases cellular motility and mitochondrial activity and regulates the gene expression profiles of molecules involved in cell adhesion, cell migration and extracellular matrix turnover. Proteomic and mass spectrometry analysis reveales an increase of cofilin, a molecule participating in actin filament dynamics. INSL3 causes a reduction in cellular proliferation, increases cellular motility in FTC-133 and downregulates transcripts for the inhibitory transcription factor Id-2H. These data demonstrate
doi:10.25673/3554 fatcat:ubbnpfoqg5fm7p6uqec37mfgjm