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DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species, and for Pyrenochaeta terrestris, which causes pink root in onions. This protocol was based on the sodium dodecyl sulfate/ phe nol method, without β-mercaptoethanol and without maceration in liquiddoi:10.4238/vol9-1gmr680 pmid:20198572 fatcat:kaeds7uicvcfrbw7pxnew4d6nu