TERATOGENICITY TESTING OF SIDDHA FORMULATION OF NILAVEMBU KUDINEER ON ZEBRAFISH (DANIO RERIO) EMBRYO

SHANMUGAPRIYA P, ELANSEKARAN S, RAMAMURTHY M, JEEVA GLADYS R, SIVARAMAN D, CHRISTIAN GJ
2019 Asian Journal of Pharmaceutical and Clinical Research  
Objective: The aim of the study was to evaluate the teratogenic effect of Nilavembu Kudineer (NVK) by testing in zebrafish embryo (Danio rerio). Methods: The study consisted of 30 embryos/culture plate/dose concentration containing a series of diluted decoction of NVK ranging from 10 μg to 640 μg/ml, and the embryo development was monitored at specific time points. The parameters such as developmental abnormality and adverse events were monitored at 24 hourly intervals for 96 h. Results: The
more » ... h. Results: The study results showed 100% hatching and survival of embryos with no significant abnormalities in the extension of study from 96 to 120 h post-fertilization examinations. Conclusion: NVK did not have teratogenic potential on testing it in various concentrations on zebrafish embryos validating its safety during pregnancy.
doi:10.22159/ajpcr.2019.v12i18.33664 fatcat:fsmjv7f46ng4zbft4lfey3zsoi