Genetic Variability of CSN1S1 Gene in Goat Populations Raised in Southeastern Region of Turkey
Güneydoğu Anadolu Bölgesi'nde Yetiştirilen Keçilerde Alfa-s1-Kazein (CSN1S1) Genindeki Genetik Çeşitlilik

2013 Kafkas Universitesi Veteriner Fakultesi Dergisi  
The objective of this study was to investigate genetic variability of CSN1S1 gene coding for alpha-s1-casein in goat populations raised in Southeastern Region of Turkey. Blood samples were collected from goats raised in Kilis(n=60), Sanliurfa (n=56), and Siirt (n=55) provinces of Turkey. From the blood samples DNA was isolated by using phenol-chloroform extraction. Genotypes of animals were determined by using polymerase chain reaction (PCR), allele specific PCR or PCR and restriction fragment
more » ... striction fragment length polymorphism methods. In Kilis and Sanliurfa populations CSN1S1 A*, B*, F and N alleles were observed, while in Siirt population only A* and B* alleles were found. Frequencies of A*, B*, F and N alleles were 0.375, 0.367, 0.017 and 0.242 in Kilis, 0.632, 0.208, 0.009 and 0.151in Sanliurfa and 0.782, 0.218, 0.000 and 0.000 in Siirt populations, respectively. CSN1S1 E and 01 alleles were not observed among the populations studied. Observed and expected genotype frequencies did not differ significantly (P>0.05). The results of this study suggested that there were sufficient genetic variability of CSN1S1 gene especially in Sanliurfa and Kilis populations in order to select individuals for different breeding purposes.
doi:10.9775/kvfd.2012.7402 fatcat:2ougebaavvhs3agrf4tblvwpvm