Association of High Proliferation in Adult T-cell Leukemia Cells With Apoptosis, and Expression of p53 Protein in Acute Type ATL

Jia Wang, Kazuhisa Hasui, Atae Utsunomiya, Xinshan Jia, Takami Matsuyama, Fusayoshi Murata
2008 Journal of Clinical and Experimental Hematopathology  
Proliferation, apoptosis and p53 protein expression in adult T-cell leukemia (ATL) cells were investigated. Twenty peripheral blood tissue specimens (PBTS) comprising 7 cases of acute type ATL, 7 cases of chronic type ATL and 6 other leukemias were examined by means of antigen retrieval and the polymer method employing anti-Ki67 antigen (MIB-1), anti-cleaved caspase-3, anti-single stranded DNA and three kinds of anti-p53 protein antibodies including DO7. Most acute and chronic cases of ATL
more » ... ded more than 10% MIB-1-positive proliferating leukemia cells and more than 1% cleaved caspase-3positive apoptotic cells. Some cells which were positive for both MIB-1 and anti-cleaved caspase-3 antibody were observed in acute type ATL. Nuclear deposition of p53 protein labeled by DO7 was often found in acute type (p < 0.05). Within the medium-sized population of ATL cell nuclei, DO7-positive ATL cells had a smaller nuclear area factor (long axis x short axis) than DO7-negative ATL cells. A few proliferating ATL cells entered apoptosis, and the appearance of a subclone of ATL cells with nuclear deposition of p53 protein labeled by DO7 characterized acute type. 〔J Clin Exp Hematopathol 48(1) : 1-10, 2008〕 Keywords: adult T-cell leukemia (ATL), peripheral blood tissue specimen (PBTS), proliferation, apoptosis, double immunostaining MATERIALS AND METHODS Twenty specimens were examined in this study. Specimens were formalin-fixed and paraffin-embedded PBTS from patients with ATL acute type (n = 7), ATL chronic type 1
doi:10.3960/jslrt.48.1 pmid:18434687 fatcat:67bsjonzzzehxeujs2cwohzgci