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Crustacean reproduction is controlled by number of hormonal and neuronal factors. The present investigation has been undertaken to observe the influence of β-estradiol hormone and eyestalk ablation on the ovarian maturation in relation to protein changes in the ovary and hepatopancreas of fresh water crab, Barytelphusa cunicularis. Protein levels were estimated by Lowry et al., (1951); Folin phenol reagent method and were calculated on dry weight basis and expressed as % mg. Base control crabfatcat:7fizdm45evfmrl5zyupvbslsmm