Comparison of the in vitro activity of novel and established nitrification inhibitors applied in agriculture: challenging the effectiveness of the currently available compounds [article]

Evangelia S. Papadopoulou, Eleftheria Bachtsevani, Eleni Lampronikou, Eleni Adamou, Afroditi Katsaouni, Cecile Thion, Sotirios Vasileiadis, Urania Menkissoglu- Spiroudi, Graeme W. Nicol, Dimitrios G. Karpouzas
2020 bioRxiv   pre-print
Nitrification inhibitors (NIs) reduce nitrogen fertilizer losses from agricultural ecosystems. Currently available NIs appear to selectively inhibit ammonia-oxidizing bacteria (AOB), while their impact on other groups of nitrifiers is limited. Ethoxyquin (EQ), a preservative shown to inhibit ammonia-oxidizers (AO) in soil, is rapidly transformed to 2,6-dihydro-2,2,4-trimethyl-6-quinone imine (QI) and 2,4-dimethyl-6-ethoxy-quinoline (EQNL). We compared the inhibitory potential of EQ and its
more » ... of EQ and its derivatives in vitro with other established NIs (DCD, nitrapyrin, DMPP) by evaluating their impact on the activity and growth of five soil-derived strains (two AOB (Nitrosomonas europaea, Nitrosospira multiformis), two ammonia-oxidizing archaea (AOA) (Candidatus Nitrosocosmicus franklandus, CandidatusNitrosotalea sinensis), and one nitrite-oxidizing bacterium (NOB) (Nitrobacter sp.)). NIs degradation was also determined. AOA were more sensitive than AOB or NOB to EQ and its derivatives. Despite its transient character, QI was primarily responsible for AO inhibition by EQ, and the most potent NI against AOA. For AOB, QI was more potent than DCD but less than nitrapyrin and DMPP. AOA and NOB showed higher tolerance to the persistent compounds DCD and DMPP. Our findings benchmark the activity range of known and novel NIs with practical implications for their use, and the development of novel NIs with broad or complementary activity against all AO.
doi:10.1101/2020.04.07.023168 fatcat:chdwu6yr6bhjnhhncsggowhqz4