Mycobiota and aflatoxin B1 in poultry feeds
Biotechnology in Animal Husbandry
In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B 1 (AFB 1 ) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 10 2 (2 log CFU g -1
... 0 2 (2 log CFU g -1 ) to 1.83 x 10 5 CFU g -1 (5.26 log CFU g -1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 10 2 to 4.8 x 10 4 CFU g -1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 10 4 to 1.83 x 10 5 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB 1 was detected in concentrations from 1.34 to 18.29 g kg -1 , with an average of 4.47 and 4.56 g kg -1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB 1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.