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Structure of tRNA splicing enzyme Tpt1 illuminates the mechanism of RNA 2′-PO4 recognition and ADP-ribosylation
2019
Nature Communications
Tpt1 is an essential agent of fungal tRNA splicing that removes the 2′-PO 4 at the splice junction generated by fungal tRNA ligase. Tpt1 catalyzes a unique two-step reaction whereby the 2′-PO 4 attacks NAD + to form an RNA-2′-phospho-ADP-ribosyl intermediate that undergoes transesterification to yield 2′-OH RNA and ADP-ribose-1″,2″-cyclic phosphate products. Because Tpt1 is inessential in exemplary bacterial and mammalian taxa, Tpt1 is seen as an attractive antifungal target. Here we report a
doi:10.1038/s41467-018-08211-9
fatcat:3d4flocpufcl7fhrdn5dezkciu