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Self-Annealing of Sendai Virus RNA
1974
Journal of Virology
Both complementary strands are found in 50S Sendai virion RNA. 50S Sendai virion RNA has been shown to consist of unequal amounts of a single population of plus and minus strands by annealing studies. Preparation of [3H Jds-Sendai RNA core. [lH ]50S Sendai RNA (93.5 Mg, 1.03 x 106 counts/min, prepared as described in Fig. 1 ) was annealed in 0.75 ml of 50% formamide and 3 x buffer A (1 x buffer A: 150 mM NaCl, 20 mM Tris-Cl, pH 7.4, 1 mM EDTA) at 37 C for 90 min. The RNA was precipitated by the
doi:10.1128/jvi.14.1.33-39.1974
fatcat:hpoxeuzyubddbjtvqha6enga6e