Internet Archive Scholar

Toxicity assessment of Syzygium jambos and Solanum guaraniticum hydroethanolic leaf extracts thru Artemia salina lethality and spleen lymphocyte cytotoxicity tests

Gabriela Bonfanti-Azzolin, Andressa Leal Zambra, Bruna Morgan da Silva, Karine Santos De Bona, Paula Eliete Rodrigues Bitencourt, Lariane Cargnelutti, Raphaela Maleski Borges, Mayara L. De Freitas, Ricardo Brandão, Josiane Woutheres Bortolotto, Aline Augusti Boligon, Aline S. Pigatto (+2 others)
2019 International journal for innovation education and research  

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (769.3 kB)
https://web.archive.org/web/20191211031906/https://ijier.net/index.php/ijier/article/download/1758/1173

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL. The file type is application/pdf.

The aim of this study was to investigate the toxicity of Syzygium jambos and Solanum guaraniticum leaf extracts, through Artemia salina lethality testing and cytotoxicity evaluations in rat spleen lymphocytes, using methyl tetrazolium (MTT), neutral red uptake (NRU), trypan blue dye exclusion, and lactate dehydrogenase (LDH) leakage assays, besides by their in vitro effects on acetylcholinesterase (AChE) activity. The LC50 calculated in the A. salina bioassay demonstrated that both extracts
more » ... t well be toxic. Solanum guaraniticum presented cytotoxic effects against lymphocytes, as demonstrated by viable cells count reductions, NR uptake, and increased LDH leakage. Syzygium jambos appears to present immunomodulatory properties, increasing lymphocyte mitochondrial activity and inhibiting AChE activity. The results demonstrated the possible harmful effects of these vegetal preparations and may be helpful in therapeutic decisions and future studies with respect to the toxicology of these extracts, when used as phytotherapeutic medicines.
doi:10.31686/ijier.vol7.iss10.1758 fatcat:wvfcgq6tobbolnccdajjv3ncyi
Szczepanski
Citation

Gabriela Bonfanti-Azzolin, Andressa Leal Zambra, Bruna Morgan da Silva, Karine Santos De Bona, Paula Eliete Rodrigues Bitencourt, Lariane Cargnelutti, Raphaela Maleski Borges, Mayara L. De Freitas, Ricardo Brandão, Josiane Woutheres Bortolotto, Aline Augusti Boligon, Aline S. Pigatto, Maria Beatriz Moretto, Thissiane de Lima Gonçalves. "Toxicity assessment of Syzygium jambos and Solanum guaraniticum hydroethanolic leaf extracts thru Artemia salina lethality and spleen lymphocyte cytotoxicity tests." International journal for innovation education and research 7.10 (2019) 133-143