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The fish industry generates high volume of waste from fish oil that can have the extraction of its lipids used as nutraceuticals and foods. The objective of this study was to produce unsaturated fatty acids from industrialized fish oil by means of a differentiated hydrolysis process. The samples used were crude fish oil obtained from Campestre industry and characterized through physicalchemical parameters, according to ADCS: acidity, peroxide, saponification, iodine and percentage of free fattydoi:10.1590/1678-457x.6583 fatcat:advxwhl5vzg6hip235uefr5o7i